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Influence of Moringa oleifera on pharmacokinetic disposition of rifampicin using HPLC‐PDA method: a pre‐clinical study
Author(s) -
Pal Anirban,
Bawankule Dnyaneshwar Umrao,
Darokar Mahendra Pandurang,
Gupta Subhash Chandra,
Arya Jai Shankar,
Shanker Karuna,
Gupta Madan Mohan,
Yadav Narayan Prasad,
Singh Khanuja Suman Preet
Publication year - 2011
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1494
Subject(s) - rifampicin , pharmacokinetics , chemistry , bioavailability , pharmacology , high performance liquid chromatography , drug , plasma concentration , oral administration , antibiotics , chromatography , medicine , biochemistry
Abstract The influence of active fraction isolated from pods of an indigenous plant, Moringa oleifera ( MoAF ) was studied on the pharmacokinetic profile of the orally administered frontline anti‐tuberculosis drug rifampicin (20 mg/kg b.w.) in Swiss albino mice. The antibiotic rifampicin alone and in combination with MoAF (0.1 mg/kg b.w.) was administered orally and heparanized blood samples were collected from the orbital plexus of mice for plasma separation at 0, 1, 2, 3, 4 and 5 h, post treatment. Plasma rifampicin concentration, pharmacokinetic parameters and drug metabolizing enzyme (cytochrome P‐450) activity were determined. The pharmacokinetic data revealed that MoAF ‐treated animals had significantly increased rifampicin plasma concentration, C max , K el , t ½(a) , t ½(el) , K a and AUC as well as inhibited rifampicin‐induced cytochrome P‐450 activity. In conclusion, the result of this study suggested that the bioavailability‐enhancing property of MoAF may help to lower the dosage level and shorten the treatment course of rifampicin. Copyright © 2010 John Wiley & Sons, Ltd.