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An analytical method for prohexadione in Chinese cabbage and apple
Author(s) -
Choi JeongHeui,
Yoon HaeJung,
Do JungAh,
Park YongChun,
Kim Jae Hun,
Choi Dongmi
Publication year - 2011
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1472
Subject(s) - chemistry , chromatography , residue (chemistry) , brassica , acetonitrile , electrospray ionization , malus , analytical chemistry (journal) , mass spectrometry , horticulture , biochemistry , biology
A residual determination method as a regulatory residue method was developed using HPLC‐UVD for prohexadione residues in Chinese cabbage ( Brassica pekinensis ) and apple ( Malus domestica ). The developed method consisted of solid–liquid extraction with acidic acetonitrile and ion‐suppression liquid–liquid partitioning, followed by anion exchange cartridge cleanup. The limits of detection and quantitation for the method were 0.005 and 0.02 mg/kg, respectively. The method gave good linearity in the range of 0.02–2.5 mg/kg. Accuracy and precision ranged from 84.1 to 94.1% and from 2.4 to 6.9%, respectively. Additionally, the confirmative conditions of LC‐MS/MS for prohexadione were set in negative electrospray ionization mode with transitions of m/z 211.4 → 167.5 and m/z 211.4 → 123.5 in the selected reaction monitoring mode. The applicability of the method was demonstrated by analyzing real samples collected from local markets in Seoul, Republic of Korea. This developed method fully deserves consideration in accordance with its sensitivity, accuracy and precision required for residue analysis of prohexadione in Chinese cabbages and apples. Copyright © 2010 John Wiley & Sons, Ltd.