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Ionic liquid aqueous solvent‐based microwave‐assisted hydrolysis for the extraction and HPLC determination of myricetin and quercetin from Myrica rubra leaves
Author(s) -
Du FuYou,
Xiao XiaoHua,
Li GongKe
Publication year - 2011
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1470
Subject(s) - chemistry , hydrolysis , myricetin , ionic liquid , chromatography , myrica rubra , quercetin , extraction (chemistry) , aqueous solution , solvent , high performance liquid chromatography , organic chemistry , botany , kaempferol , catalysis , biology , antioxidant
An ionic liquid aqueous solvent‐based microwave‐assisted hydrolysis (ILAS‐MAH) approach was proposed for the rapid extraction and accurate determination of myricetin and quercetin from Myrica rubra ( M . rubra ) leaves for the first time. The effects of the ionic liquid class and concentration, liquid–solid ratio, hydrolysis temperature and time were investigated to obtain the optimal ILAS‐MAH conditions. The optimized conditions were 2.0 mol/L [bmin][HSO4] or 2.5 mol/L acidified [bmin]Br solution, liquid–solid ratio 30:1 (mL : g), hydrolysis temperature 70°C and hydrolysis time 10 min. Under these conditions, the recoveries of myricetin and quercetin were in the range of 86.3–107.3% with relative standard deviation lower than 5.8%. Compared with conventional heating hydrolysis and regular MAH, the proposed approach reduced hydrolysis time and improved yields. The mechanism of ILAS‐MAH was also investigated. Copyright © 2010 John Wiley & Sons, Ltd.