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Metabolic analysis of rhubarb extract by rat intestinal bacteria using liquid chromatography–tandem mass spectrometry
Author(s) -
Song Rui,
Xu Lei,
Xu Fengguo,
Dong Haijuan,
Tian Yuan,
Zhang Zunjian
Publication year - 2011
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1467
Subject(s) - chemistry , anthraquinones , chromatography , emodin , glycoside , biotransformation , tandem mass spectrometry , bacteria , anthraquinone , mass spectrometry , liquid chromatography–mass spectrometry , biochemistry , organic chemistry , enzyme , botany , genetics , biology
Through investigation of the metabolism of rhubarb extract by rat intestinal bacteria, a total of 14 components in rhubarb extract were found to be biotransformed. These components included aloe‐emodin‐ O‐ glucosides, emodin‐ O‐ glucosides, chrysophanol‐ O‐ glucosides, physcion‐ O‐ glucosides and the corresponding aglycones. Rhein also could be biotransformed by rat intestinal bacteria. Twelve major metabolites were detected in the incubation sample. Under ESI tandem mass conditions, the sequential fragmentation patterns of [M H] ‐ ions were similar to those of free anthraquinones, thus allowing the rapid identification of the metabolites formed in incubation samples. The results suggested that the proposed hydrolysis of glycoside group followed by hydrogenation in quinoid moiety and/or further acetylation was the major biotransformation pathway for these anthraquinone glycosides by rat intestinal bacteria.