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Liquid chromatography tandem mass spectrometry method for determination of N ‐acetylcysteine in human plasma using an isotope‐labeled internal standard
Author(s) -
Lu Chuan,
Liu Gangyi,
Jia Jingying,
Gui Yuzhou,
Liu Yanmei,
Zhang Mengqi,
Liu Yun,
Li Shuijun,
Yu Chen
Publication year - 2011
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1465
Subject(s) - chemistry , chromatography , tandem mass spectrometry , mass spectrometry , pharmacokinetics , liquid chromatography–mass spectrometry , electrospray ionization , protein precipitation , acetylcysteine , trichloroacetic acid , selected reaction monitoring , high performance liquid chromatography , bioequivalence , antioxidant , pharmacology , biochemistry , medicine
A liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method was developed to determine total N ‐acetylcysteine in human plasma. Mass spectrometric detection was achieved in positive electrospray ionization and multiple reaction monitoring mode. The mass transition pairs of N ‐acetylcysteine and the isotope‐labeled internal standard d3‐ N ‐acetylcysteine were 164 → 122 and 167 → 123, respectively. The method was linear over the range of 10–5000 ng/mL in human plasma. The adoption of trichloroacetic acid significantly enhanced the extraction recovery. The blank matrix was screened to minimize the influence of endogenous N ‐acetylcysteine. After being fully validated, the method was successfully applied to the pharmacokinetic and bioequivalent study of N ‐acetylcysteine after oral administration of 600 mg tablets to 24 healthy Chinese volunteers. Copyright © 2010 John Wiley & Sons, Ltd.