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Bufferized solvent extraction and HPLC fluorometric detection method for sarafloxacin in pig and chicken muscles
Author(s) -
Choi JeongHeui,
Na Tae Woong,
Rouf Mamun Md. Iqbal,
Abd ElAty A. M.,
Shin EunHo,
Park JongHyouk,
Shim JaeHan
Publication year - 2011
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1463
Subject(s) - chemistry , chromatography , residue (chemistry) , high performance liquid chromatography , solvent , extraction (chemistry) , detection limit , hexane , acetonitrile , biochemistry
In this study, a method for the detection of sarafloxacin in pig and chicken muscles was developed using HPLC‐FLD as a regulatory residue technique. Good extraction efficiency was achieved using a mixture of 1% orthophosphoric acid–0.2 m MgCl 2 in water and acetonitrile as an extraction solvent, and n ‐hexane partitioning and centrifugation for cleanup was used in the absence of dehydration. Specificity, linearity, detection and quantification limits, recovery, accuracy and precision were all validated, and all results were sufficient for the SARA regulatory residue method in pig and chicken muscles. The method developed and described herein was not only simple but also reliable, and was applied to market samples to determine their residue contents. Copyright © 2010 John Wiley & Sons, Ltd.