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A simple and sensitive HPLC‐UV method for the determination of ponicidin in rat plasma and its application in a pharmacokinetic study
Author(s) -
Li Xiaotian,
Hou Wenjing,
Song Jingjing,
Liu Hongmin,
Song Shumei,
Zhang Lingchun,
Shi Yingying
Publication year - 2011
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1456
Subject(s) - chromatography , chemistry , phosphoric acid , analyte , extraction (chemistry) , high performance liquid chromatography , relative standard deviation , methanol , pharmacokinetics , detection limit , ethyl acetate , analytical chemistry (journal) , medicine , organic chemistry
A simple, rapid, selective and sensitive HPLC‐UV method has been developed and validated for the determination of ponicidin in rat plasma. The analyte was extracted from rat plasma by liquid–liquid extraction with ethyl acetate as the extraction solvent. The LC separation was performed on a Zorbax Eclipse XDB C 18 analytical column (150 × 4.6 mm i.d., 5 µm) with an isocratic mobile phase consisting of methanol–water–phosphoric acid (45:55:0.01, v/v/v) at a flow rate of 1.0 mL/min. There was a good linearity over the range of 0.1–25 µg/mL ( r = 0.9995) with a weighted (1/ C 2 ) least square method. The lower limit of quantification was proved to be 0.1 µg/mL. The accuracy was within ±10.0% in terms of relative error and the intra‐ and inter‐day precisions were less than 9.1% in terms of relative standard deviation. After validation, the method was successfully applied to characterize the pharmacokinetics of ponicidin in rats. Copyright © 2010 John Wiley & Sons, Ltd.