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A liquid chromatography–tandem mass spectrometry method for the simultaneous quantification of escin Ia and escin Ib in human plasma: application to a pharmacokinetic study after intravenous administration
Author(s) -
Liu Lidong,
Wu Xiujun,
Wu Dan,
Wang Yingwu,
Li Pengfei,
Sun Yantong,
Yang Yan,
Gu Jingkai,
Cui Yimin
Publication year - 2010
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1441
Subject(s) - chemistry , chromatography , selected reaction monitoring , analyte , mass spectrometry , pharmacokinetics , liquid chromatography–mass spectrometry , extraction (chemistry) , tandem mass spectrometry , pharmacology , medicine
Abstract A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method was developed and validated for simultaneous quantification of escin Ia and escin Ib in human plasma. After a solid‐phase extraction (SPE), the analytes were separated on a Zorbax Extend C 18 column by isocratic elution with a mobile phase of methanol–acetonitrile–10 m m ammonium acetate (27:27:46, v/v/v) at a flow rate of 1.0 mL/min and analyzed by mass spectrometry in the positive ion multiple reaction monitoring mode. The precursor to product ion transitions of m / z 1131.8 → 807.6 was used to quantify escin Ia and escin Ib. Good linearity was achieved over a wide range of 2.00–900 ng/mL for escin Ia and 1.50–662 ng/mL for escin Ib. The intra‐ and inter‐day precisions (as relative standard deviation) were less than 11% for each QC level of escin Ia and escin Ib. The accuracies (as relative error) were within ±5.27% for escin Ia and within ±4.07% for escin Ib. The method was successfully employed in a pharmacokinetic study after a single intravenous infusion administration of sodium aescinate injection containing 10 mg escin to each of the 10 healthy volunteers. Copyright © 2010 John Wiley & Sons, Ltd.