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Analysis of glycosaminoglycan‐derived disaccharides in biologic samples by capillary electrophoresis and protocol for sequencing glycosaminoglycans
Author(s) -
Lamari F. N.,
Militsopoulou M.,
Mitropoulou T. N.,
Hjerpe A.,
Karamanos N. K.
Publication year - 2002
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.144
Subject(s) - glycosaminoglycan , glycomics , chemistry , capillary electrophoresis , dermatan sulfate , keratan sulfate , disaccharide , heparan sulfate , sulfation , chondroitin sulfate , biochemistry , chondroitin , proteomics , chromatography , glycan , glycoprotein , gene
Glycosaminoglycans are biologically significant carbohydrates which either as free chains (hyaluronan) or constituents of proteoglycans (chondroitin/dermatan sulfates, heparin, heparan sulfate and keratan sulfate) participate and regulate several cellular events and (patho)physiological processes. Capillary electrophoresis, due to its high resolving power and sensitivity, has been successfully used for the analysis of glycosaminoglycans. Determination of compositional characteristics, such as disaccharide sulfation pattern, is a useful prerequisite for elucidating the interactions of glycosaminoglycans with matrix effective molecules and, therefore, essential in understanding the biological functions of proteoglycans. The interest in the field of characterization of such biologically important carbohydrates is soaring and advances in this field will signal a new revolution in the area of glycomics equivalent to that of genomics and proteomics. This review focuses on the capillary electrophoresis methods used to determine the disaccharide pattern of glycosaminoglycans in various biologic samples as well as advances in the sequence analysis of glycosaminoglycans using both chromatographic and electrophoretic techniques. Copyright © 2002 John Wiley & Sons, Ltd.