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Enantioseparation of dansyl amino acids by HPLC on a monolithic column dynamically coated with a vancomycin derivative
Author(s) -
Pittler Elfriede,
Schmid Martin G.
Publication year - 2010
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1430
Subject(s) - chemistry , enantiomer , chromatography , high performance liquid chromatography , elution , alkyl , phase (matter) , derivative (finance) , analyte , vancomycin , chiral stationary phase , covalent bond , organic chemistry , biology , bacteria , financial economics , economics , genetics , staphylococcus aureus
In this work a chiral stationary phase was prepared by dynamically coating a monolithic reversed‐phase HPLC column with a vancomycin‐derivative as chiral selector. A hydrophobic alkyl‐chain was attached to the vancomycin molecule, providing the immobilization of the chiral selector on the reversed‐phase material. Dansyl amino acids were chosen as model analytes for testing the separation power of the dynamically coated phase. All investigated compounds were separated into their enantiomers. Compared with a conventionally packed vancomycin‐CSP, a reversal of the enantiomer elution order was obtained.