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Characterization of anti‐Coxsackie virus B3 constituents of Radix Astragali by high‐performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry
Author(s) -
Ye Guan,
Tang YiHong,
Xia GuangXin,
Sun ZhaoLin,
Li ZhiXiong,
Huang ChengGang
Publication year - 2010
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1400
Subject(s) - chemistry , chromatography , radix (gastropod) , electrospray ionization , tandem mass spectrometry , high performance liquid chromatography , chemical constituents , mass spectrometry , glucoside , retention time , liquid chromatography–mass spectrometry , medicine , botany , alternative medicine , pathology , biology
To profile the anti‐Coxsackie virus B3 constituents of Radix Astragali, an HPLC‐DAD‐MS n analytical method, combined with an in vivo test, has been developed to identify the constituents of the active part, which has been demonstrated to have potency to inhibit the proliferation of virus in cardiac muscle, alleviate infraction in heart and elevate the survival rate of the animal. By comparing their retention time and MS data with those obtained from the authentic compounds and the published data, a total of 19 compounds, including 11 isoflavonoids and eight saponins, were identified, among which one pterocarpane glucoside was reported for the first time. The present study provides an approach to rapidly screening bioactive constituents in traditional Chinese medicines. Copyright © 2010 John Wiley & Sons, Ltd.