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Rapid high‐performance liquid chromatographic assay of dorzolamide in rabbit aqueous humor
Author(s) -
Maltese Adriana,
Bucolo Claudio
Publication year - 2002
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.138
Subject(s) - chemistry , chromatography , rabbit (cipher) , dorzolamide , high performance liquid chromatography , aqueous solution , aqueous humor , organic chemistry , ophthalmology , medicine , timolol , intraocular pressure , statistics , mathematics
A rapid high‐performance liquid chromatographic method using a C 18 reversed‐phase column (Hypersil ODS) with UV detection at 254 nm and a simple pre‐treatment of samples is presented for the analysis of dorzolamide, a carbonic anhydrase inhibitor, in rabbit aqueous humor. A water solution containing 2% ZnSO 4 ·7H 2 O was used to deproteinize aqueous humor samples. The mobile phase consisted of 7% CH 3 CN and 93% of a solution containing 1% TEA adjusted to pH = 3.5 with H 3 PO 4 . Paracetamol was found to be a suitable internal standard. The standard curves were linear in the detection range. The precision and the accuracy were <5% for both intra‐ and inter‐day assays. Copyright © 2002 John Wiley & Sons, Ltd.