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Determination of eflornithine enantiomers in plasma by precolumn derivatization with o ‐phthalaldehyde‐ N ‐acetyl‐ l ‐cysteine and liquid chromatography with UV detection
Author(s) -
JanssonLöfmark R.,
Römsing S.,
Albers E.,
Ashton M.
Publication year - 2010
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1361
Subject(s) - chemistry , chromatography , derivatization , o phthalaldehyde , enantiomer , high performance liquid chromatography , eflornithine , biochemistry , enzyme , organic chemistry , spermidine
A bioanalytical method for indirect determination of eflornithine enantiomers in 75 μL human plasma has been developed and validated. l ‐ and d ‐eflornithine were derivatized with o ‐phthalaldehyde and N ‐acetyl‐L‐cysteine to generate diastereomers which were separated on two serially connected Chromolith Performance columns (RP‐18e 100 × 4.6 mm i.d.) by a isocratic flow followed by a gradient flow for elution of endogenous compounds. The diastereomers were detected with UV (340 nm). The between‐day precisions for L‐ and D‐eflornithine in plasma were 8.4 and 2.3% at 3 μ m , 4.0 and 5.1% at 400 μ m , and 2.0 and 3.7% at 1000 μ m . The lower limit of quantification was determined to be 1.5 μ m , at which precision was 14.9 and 9.9% for L‐ and D‐eflornithine, respectively. Copyright © 2009 John Wiley & Sons, Ltd.