Premium
High‐performance liquid chromatographic analysis: applications to nutraceutical content and urinary disposition of oxyresveratrol in rats
Author(s) -
Bertram Rebecca M.,
Takemoto Jody K.,
Remsberg Connie M.,
VegaVilla Karina R.,
Sablani Shyam,
Davies Neal M.
Publication year - 2010
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1320
Subject(s) - chromatography , chemistry , formic acid , detection limit , calibration curve , high performance liquid chromatography , extraction (chemistry) , quantitative analysis (chemistry) , urine , chromatographic separation , linear range , biochemistry
A high‐performance liquid chromatographic (HPLC) method was developed for the analysis of the stilbene, oxyresveratrol. This method involves the use of a Luna® C 18 column with ultraviolet detection at 320 nm. The mobile phase consisted of acetonitrile, water and formic acid (30 : 70 : 0.04 v/v) with a flow rate of 0.6 mL/min. The calibration curves were linear over the range of 0.5–100.0 μg/mL. The mean extraction efficiency was between 98.9 and 109%. The precision of the assay was 0.069–18.4% (RSD%), and within 20% at the limit of quantitation (0.5 μg/mL). The bias of the assay was <15% and within 15% at the limit of quantitation. This assay was successfully applied to pre‐clinical pharmacokinetic samples from rat urine and to nutraceutical product analysis. Copyright © 2009 John Wiley & Sons, Ltd.