Premium
Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography‐mass spectrometry
Author(s) -
Hu Yong Mei,
Wang Yi Tao,
Sze Stephen C. W.,
Tsang K. W.,
Wong H. K.,
Liu Qing,
Zhong Li Dan,
Tong Yao
Publication year - 2010
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1315
Subject(s) - decoction , chemistry , oral administration , chromatography , in vivo , pharmacology , traditional medicine , medicine , microbiology and biotechnology , biology
A simple and specific LC‐DAD‐ESI‐MS/MS method has been developed and applied for the primary investigation of the chemical constituents absorbed or metabolized in vivo , after the rat oral administration of Erxian Decoction (EXD), a Chinese medicine prescription for menopausal syndromes. Through the online ESI‐MS n analysis, a total of 35 compounds have been identified or tentatively characterized from the seven tested samples, and 13 of them were unambiguously identified through a direct comparison of the retention time, UV spectra and MS n fragmentation patterns with the authentic ones. The results showed that 21 compounds were detected from rat plasma, 20 compounds were detected from rat kidneys and adrenal glands, 19 compounds were detected from rat ovaries, 12 compounds were found in rat intestines, nine compounds were identified from rat livers and nine compounds were detected from rat brains at certain time points after oral administration of the effective EXD fraction. Copyright © 2009 John Wiley & Sons, Ltd.