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LC–MS/MS method for the simultaneous determination of ethyl gallate and its major metabolite in rat plasma
Author(s) -
Gao Shouhong,
Zhan Qin,
Li Jingxian,
Yang Qi,
Li Xia,
Chen Wansheng,
Sun Lianna
Publication year - 2010
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1314
Subject(s) - chemistry , chromatography , protein precipitation , gallic acid , ethyl acetate , formic acid , selected reaction monitoring , gallate , metabolite , active metabolite , ammonium acetate , methyl gallate , propyl gallate , pharmacokinetics , mass spectrometry , acetonitrile , electrospray ionization , tandem mass spectrometry , nuclear chemistry , high performance liquid chromatography , organic chemistry , biochemistry , antioxidant , medicine
A simple, rapid and sensitive liquid chromatography–tandem mass spectroscopy (LC–MS/MS) method was developed and validated for the determination of ethyl gallate, a pharmacologically active constituent isolated from Lagerstroemia speciosa (Linn.) Pers. This method was used to examine the pharmacokinetics of ethyl gallate and its major metabolite gallic acid in rat plasma using propyl gallate as an internal standard. After precipitation of the plasma proteins with acetonitrile, the analytes were separated on a Zorbax SB‐C 18 column (3.5 μm, 2.1 × 50 mm) with an isocratic mobile phase consisted of methanol–acetonitrile–10 mM ammonium acetate (10 : 25 : 65, v/v/v) containing 0.1% formic acid at a flow rate of 0.25 mL/min. The Agilent G6410A triple quadrupole LC/MS system was operated under the multiple‐reaction monitoring mode using the electrospray ionization technique in negative mode. The lower limits of quantification of gallic acid and ethyl gallate of the method were 0.5 and 1.0 ng/mL. The intra‐day and inter‐day accuracy and precision of the assay were less than 8.0%. This method has been applied successfully to a pharmacokinetic study involving the intragastric administration of ethyl gallate to rats. Copyright © 2009 John Wiley & Sons, Ltd.