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Determination of dimethyl sulfoxide and dimethyl sulfone in urine by gas chromatography–mass spectrometry after preparation using 2,2‐dimethoxypropane
Author(s) -
Takeuchi Akito,
Yamamoto Shinobu,
Narai Rie,
Nishida Manami,
Yashiki Mikio,
Sakui Norihiro,
Namera Akira
Publication year - 2010
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1313
Subject(s) - chemistry , dimethyl sulfoxide , chromatography , mass spectrometry , urine , gas chromatography–mass spectrometry , hydrochloric acid , gas chromatography , sulfoxide , sulfone , detection limit , organic chemistry , biochemistry
A method for routinely determination of dimethyl sulfoxide (DMSO) and dimethyl sulfone (DMSO 2 ) in human urine was developed using gas chromatography–mass spectrometry. The urine sample was treated with 2,2‐dimethoxypropane (DMP) and hydrochloric acid for efficient removal of water, which causes degradation of the vacuum level in mass spectrometer and shortens the life‐time of the column. Experimental DMP reaction parameters, such as hydrochloric acid concentration, DMP–urine ratio, reaction temperature and reaction time, were optimized for urine. Hexadeuterated DMSO was used as an internal standard. The recoveries of DMSO and DMSO 2 from urine were 97–104 and 98–116%, respectively. The calibration curves showed linearity in the range of 0.15–54.45 mg/L for DMSO and 0.19–50.10 mg/L for DMSO 2 . The limits of detection of DMSO and DMSO 2 were 0.04 and 0.06 mg/L, respectively. The relative standard deviations of intra‐day and inter‐day were 0.2–3.4% for DMSO and 0.4–2.4% for DMSO 2 . The proposed method may be useful for the biological monitoring of workers exposed to DMSO in their occupational environment. Copyright © 2009 John Wiley & Sons, Ltd.