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Simple and sensitive HPLC method for determination of amrubicin and amrubicinol in human plasma: application to a clinical pharmacokinetic study
Author(s) -
Ando Reiko,
Makino Yoshinori,
Tamura Tomohide,
Yamamoto Noboru,
Nishigaki Rena,
Kimura Takehiro,
Yokote Nobuaki,
Yamamoto Hiroshi
Publication year - 2010
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1289
Subject(s) - chemistry , chromatography , protein precipitation , high performance liquid chromatography , pharmacokinetics , metabolite , analytical chemistry (journal) , pharmacology , medicine , biochemistry
A simple and sensitive high‐performance liquid chromatographic (HPLC) method was developed for determination of amrubicin and its metabolite amrubicinol in human plasma. After protein precipitation with methanol without evaporation procedure, large volume samples were injected and separated by two monolithic columns with a guard column. The mobile phase consisted of tetrahydrofuran–dioxane–water (containing 2.3 mM acetic acid and 4 mM sodium 1‐octanesulfonate; 2:6:15, v/v/v). Wavelengths of fluorescence detection were set at 480 nm for excitation and 550 nm for detection. Under these conditions, linearity was confirmed in the 2.5–5000 ng/mL concentration range of both compounds. The intra‐ and inter‐day precision and intra‐ and inter‐day accuracy for both compounds were less than 10%. The method was successfully applied to a clinical pharmacokinetic study of amrubicin and amrubicinol in cancer patients. Copyright © 2009 John Wiley & Sons, Ltd.