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Development of a fluorimetric detection method for cinnabarinic acid using ortho ‐tolyl hydrazine as the derivatization reagent
Author(s) -
Iizuka Hideaki,
Obata Kensaku,
Yajima Takehiko,
Ichiba Hideaki,
Fukushima Takeshi
Publication year - 2010
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1281
Subject(s) - chemistry , derivatization , reagent , detection limit , chromatography , hydrazine (antidepressant) , high performance liquid chromatography , moiety , fluorescence , derivative (finance) , organic chemistry , physics , quantum mechanics , financial economics , economics
A fluorimetric detection method for one of the tryptophan metabolites, cinnabarinic acid (CA), which has recently been reported to have the ability to induce apoptosis in thymocytes, was developed using o ‐tolyl hydrazine (TH) as the derivatization reagent. The carbonyl group at position 3 in CA was tagged with the hydrazino moiety of TH at 100°C for 30 min, and the generated derivative, CA tagged with TH, fluoresced at 412 nm with a 316 nm excitation wavelength. The CA tagged with TH was separated on a reversed‐phase HPLC and detected fluorometrically. The relative standard deviation was in the range of 1.1–8.9% ( n = 3), and the detection limit was approximately 12?fmol (signal‐to‐noise ratio, 3). The proposed HPLC method can be useful for the sensitive detection of CA. Copyright © 2009 John Wiley & Son, Ltd.