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Disposition of triazolam in the rat by brain microdialysis and semi‐micro column high‐performance liquid chromatography with UV absorbance detection
Author(s) -
Nakashima Kenichiro,
Yamamoto Kazumi,
AlDirbashi Osama Y.,
Nakashima Mihoko N.
Publication year - 2002
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.128
Subject(s) - triazolam , microdialysis , chemistry , chromatography , high performance liquid chromatography , reproducibility , column chromatography , extracellular , benzodiazepine , biochemistry , receptor
A semi‐micro column high‐performance liquid chromatography with ultraviolet detection for the determination of triazolam is described. The method was applied to determine plasma and brain microdialysate concentrations of triazolam after single intravenous bolus of 2.5 mg/kg to rat. The separation was achieved on a 250 × 1.5 mm i.d. C 18 column and the column effluent was monitored at 222 nm. The detection limits at a signal‐to‐noise ratio of 3 obtained using spiked plasma and artificial cerebrospinal fluid were 2.1 and 0.7 ng/mL, respectively. The intra‐ and inter‐day reproducibility of the present method were satisfactory with the highest relative standard deviation of 9.1 ( n ≥ 5). The present method was successfully applied to study the disposition of triazolam in rat ( n = 5) by analyzing plasma and brain microdialysate samples. Copyright © 2002 John Wiley & Sons, Ltd.