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Metabonomic profiling of liver metabolites by gas chromatography–mass spectrometry and its application to characterizing hyperlipidemia
Author(s) -
Gu Shenghua,
A Jiye,
Wang Guangji,
Zha Weibin,
Yan Bei,
Zhang Ying,
Ren Hongcan,
Cao Bei,
Liu Linsheng
Publication year - 2010
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1279
Subject(s) - chemistry , chromatography , mass spectrometry , metabolomics , gas chromatography–mass spectrometry , metabolite profiling , gas chromatography , hyperlipidemia , metabolite , biochemistry , biology , diabetes mellitus , endocrinology
The measurement of metabolites in tissues is of great importance in metabonomic research in the biomedical sciences, providing more relevant information than is available from systemic biofluids. The liver is the most important organ/tissue for most biochemical reactions, and the metabolites in the liver are of great interest to scientists. To develop an optimized extraction method and comprehensive profiling technique for liver metabolites, organic solvents of various compositions were designed using design of experiments to extract metabolites from the liver, and the metabolites were profiled by gas chromatography/time‐of‐flight mass spectrometry (GC/TOF‐MS). The resolved peak areas were processed by principle components analysis, partial least‐squares projections to latent structures, and discriminant analysis. The results suggest the highest extraction efficiency was for methanol–water, which maximized the majority of GC/TOF‐MS responses. The optimal solvent was applied to extract metabolites in liver of hyperlipidemia hamster and the control. The GC/TOF‐MS profiles of liver metabolites showed obvious differences between hyperlipidemic hamsters and controls. A comparison of liver and serum data from the same animals identified common biomarkers and presented complementary information. Our results suggest that liver metabonomics is a valuable technique and that the combined analysis of systematic biofluids and local tissues is meaningful and complementary, recovering more comprehensive metabonomic data than either analysis alone. Copyright © 2009 John Wiley & Sons, Ltd.