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Quality control of PET radiopharmaceuticals by high‐performance liquid chromatography with tris (2,2′‐bipyridyl)ruthenium(II) electrogenerated chemiluminescence detection
Author(s) -
Nakao Ryuji,
Furutsuka Kenji,
Fukumura Toshimitsu,
Yamaguchi Masatoshi,
Suzuki Kazutoshi
Publication year - 2010
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1274
Subject(s) - chemistry , chemiluminescence , ruthenium , high performance liquid chromatography , chromatography , tris , detection limit , reproducibility , electrochemiluminescence , catalysis , organic chemistry , biochemistry
A highly sensitive reversed‐phase liquid chromatographic (HPLC) method was investigated to analyze a range of positron emission tomography (PET) radiopharmaceuticals using electrogenerated chemiluminescence (ECL) detection. ECL is based on the reaction of PET molecules with tris (2,2′‐bipyridyl)ruthenium(III) [Ru(bpy) 3 3+ ], which is generated through the on‐line electro‐oxidation of Ru(bpy) 3 2+ . In 21 different radiopharmaceuticals studied, 18 compounds could be detected with detection limits (signal‐to‐noise ratio = 3) of 0.12–72 ng/mL per 20 μL injection. Sufficient reproducibility and linearity were obtained for the quantitative determination of PET molecules in pharmaceutical fluid. This method could be successfully applied to quality control tests of PET radiopharmaceuticals with ultra‐high specific radioactivity. Copyright © 2009 John Wiley & Sons, Ltd.