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Simultaneous determination of ondansetron and tropisetron in human plasma using HPLC with UV detection
Author(s) -
Bauer Steffen,
Störmer Elke,
Kaiser Rolf,
Tremblay PierreBenoit,
Brockmöller Jürgen,
Roots Ivar
Publication year - 2002
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.125
Subject(s) - tropisetron , chemistry , chromatography , ondansetron , high performance liquid chromatography , human plasma , pharmacokinetics , pharmacology , serotonin , nausea , receptor , anesthesia , medicine , biochemistry
A rapid and sensitve HPLC method for the simultaneous quantitation of ondansetron and tropisetron, two serotonin (5‐HT) receptor antagonists frequently used in treatment and prevention of nausea and emesis, is described. The procedure involves liqid–liquid extraction of human plasma with dichloromethane coupled with reversed‐phase HPLC and UV detection. The lower limits of quantification (LOQ) were 0.62 ng/mL for ondansetron and 1.25 ng/mL or tropisetron. Intra‐ and inter‐assay coefficients of variation ranged from 1.5 to 7.5% and 5.3 to 13.7%, respectively. The sensitivity and precision were sufficient for determination of plasma concentrations after therapeutic administration of both drugs and the method can be used for the estimation of pharmacokinetic parameters. Copyright © 2002 John Wiley & Sons, Ltd.