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Quantitative determination of a novel enantiomeric tropane analog, (−)‐satropane, in biological fluids using liquid chromatography/tandem mass spectrometry
Author(s) -
Fu Jun,
Fang Chao,
Cui YongYao,
Yang LiMin,
Zhu Liang,
Feng XueMei,
Zheng PeiLi,
Lu Yang,
Chen HongZhuan
Publication year - 2009
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1220
Subject(s) - chemistry , chromatography , tropane , microdialysis , tandem mass spectrometry , liquid chromatography–mass spectrometry , mass spectrometry , detection limit , calibration curve , bioanalysis , matrix (chemical analysis) , analyte , stereochemistry , biochemistry , extracellular
A sensitive, accurate and precise liquid chromatography–tandem mass spectrometry method was developed for the determination of (−)‐satropane (3 α ‐paramethyl‐benzenesulfonyloxy‐6 β ‐acetoxy‐tropane) in rabbit aqueous humor. Since (−)‐satropane may be absorbed from the aqueous humour with resultant systemic side effects, the LC‐MS/MS method was also evaluated for its applicability in analyzing plasma samples containing this compound. (−)‐Satropane and phentolamine (the internal standard, represented as IS) were detected by multiple reaction monitoring using the transitions m/z 354–182 and 282–212, respectively. The calibration curve was linear over the ranges 2–500 and 5–1000 ng/mL, and the values of the lower limit of quantification were 2 and 5 ng/mL for the microdialysis dialysate and rat plasma samples, respectively. The intra‐day and inter‐day precision and accuracy were better than 8.6 and 6.00%, respectively, in both matrices investigated. The absolute recovery of the plasma samples was more than 76.30%. The average matrix effects of (−)‐satropane were 91.72 and 83.05% in the microdialysis dialysate and plasma samples, respectively. The validated method was successfully applied to analyze (−)‐satropane in microdialysis dialysate and rat plasma samples, and this assay has been used to quantify (‐)‐satropane in the pharmacokinetic and toxicokinetic studies in our laboratory. Copyright © 2009 John Wiley & Sons, Ltd.