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Separation of cephalosporins on thin silica gel layers impregnated with transition metal ions and by reversed‐phase TLC
Author(s) -
Bhushan Ravi,
Thiong'o G. Thuku
Publication year - 2002
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.122
Subject(s) - chemistry , silica gel , resolution (logic) , chromatography , metal ions in aqueous solution , solvent , thin layer chromatography , phase (matter) , metal , analyte , ion , thin layers , thin layer , analytical chemistry (journal) , transition metal , cephalosporin , layer (electronics) , antibiotics , organic chemistry , catalysis , materials science , composite material , artificial intelligence , computer science , biochemistry
Cephalosporin antibiotics were separated on thin layer plates impregnated with transition metal ions, Mn 2+ , Fe 2+ , Co 2+ , Ni 2+ and Cu 2+ , using different concentrations. Various solvent systems were developed for the study and were used for separation of these analytes. Impregnation was observed to have an effect on hR F values, removed tailing of analytes and improved the resolution. The results have been discussed for each metal ion and compared, and the best conditions of separation have been identified. Activation time of thin layer plates impregnated with 0.1% FeSO 4 was found to affect both hR F values and resolution of cephalosporins. New solvent systems are reported for both normal phase and reversed‐phase TLC. Copyright © 2002 John Wiley & Sons, Ltd.