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Quantitative separation of oxytocin, norfloxacin and diclofenac sodium in milk samples using capillary electrophoresis
Author(s) -
Solangi Amber R.,
Memon Saima Q.,
Mallah Arfana,
Khuhawar M. Y.,
Bhanger M. I.
Publication year - 2009
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1215
Subject(s) - chemistry , chromatography , capillary electrophoresis , norfloxacin , diclofenac sodium , calibration curve , detection limit , sodium , electrolyte , electrode , biochemistry , organic chemistry , ciprofloxacin , antibiotics
A simple, sensitive and rapid method has been developed for simultaneous separation and quantification of three different drugs: oxytocin (OT), norfloxacin (NOR) and diclofenac (DIC) sodium in milk samples using capillary electrophoresis (CE) with UV detection at 220 nm. Factors affecting the separation were pH, concentration of buffer and applied voltage. Separation was obtained in less than 9 min with sodium tetraborate buffer of pH 10.0 and applied voltage 30 kV. The separation was carried out from uncoated fused silica capillary with effective length of 50 cm with 75 µm i.d. The carrier electrolyte gave reproducible separation with calibration plots linear over 0.15–4.0 µg/mL for OT, 5–1000 µg/mL for NOR and 3–125 µg/mL for DIC. The lower limits of detection (LOD) were found to be 50 ng/mL for OT, and 1 µg/mL for NOR and DIC. The method was validated for the analysis of drugs in milk samples and pharmaceutical preparations with recovery of drugs within the range 96–100% with RSD 0.9–2.8%. Copyright © 2009 John Wiley & Sons, Ltd.