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Metabolite analysis in Curcuma domestica using various GC‐MS and LC‐MS separation and detection techniques
Author(s) -
Herebian Diran,
Choi JeongHeui,
Abd ElAty A. M.,
Shim JaeHan,
Spiteller Michael
Publication year - 2009
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1207
Subject(s) - chemistry , chromatography , curcuma , orbitrap , gas chromatography–mass spectrometry , metabolite , mass spectrometry , curcumin , methanol , chromatographic separation , hexane , high performance liquid chromatography , traditional medicine , organic chemistry , biochemistry , medicine
The metabolic profile of polar (methanol) and non‐polar (hexane) extracts of Curcuma domestica , a widely used medicinal plant, was established using various different analytical techniques, including GC‐FID, GC‐MS, HR‐GC‐MS and analytical HPLC‐ESI‐MS/MS by means of LTQ‐Orbitrap technology. The major non‐volatile curcuminoids curcumin, demethoxycurcumin and bisdemethoxycurcumin were identified when their chromatographic and precursor ion masses were compared with those of authentic standard compounds. In this paper we describe for the first time a GC/MS‐based method for metabolic profiling of the hydrophilic extract. We also identified 61 polar metabolites as TMS derivatives. Copyright © 2009 John Wiley & Sons, Ltd.