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Improved high performance liquid chromatographic determination of ginsenosides in Panax ginseng ‐based pharmaceuticals using a diol column
Author(s) -
Bonfill Mercè,
Casals Isidre,
Palazón Javier,
Mallol Anna,
Morales Carmen
Publication year - 2002
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.120
Subject(s) - chromatography , chemistry , ginseng , detection limit , ginsenoside , high performance liquid chromatography , diol , coefficient of variation , chromatographic separation , quantitative analysis (chemistry) , medicine , alternative medicine , organic chemistry , pathology
A reversed‐phase high‐performance liquid chromatographic assay for the simultaneous quantitative determination of seven ginsenosides, Rb 1 , Rb 2 , Rc, Rd, Rg 1 , Re and Rf in pharmaceutical preparations is described. Chromatographic separation was achieved in less than 20 min using a 250 × 4 mm Lichrospher, 5 µm, 100 Å diol column with detection at 203 nm. The method was validated over the range of 2.5–20 ng/µL using a 20 µL sample volume. The average accuracy at five concentrations was 90–100%, and the within‐day and between‐day precision ranged from 1 to 7% expressed as coefficient of variation. The detection limit and the quantitation limit of the method were 20 and 50 ng injected for each ginsenoside, respectively. Copyright © 2002 John Wiley & Sons, Ltd.