Reversed‐phase liquid chromatographic determination of enantiomers of atenolol in rat plasma using derivatization with Marfey's reagent | Zendy

Bhushan Ravi | Zendy; Tanwar Shivani | Zendy

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Reversed‐phase liquid chromatographic determination of enantiomers of atenolol in rat plasma using derivatization with Marfey's reagent

Author(s) -

Bhushan Ravi,

Tanwar Shivani

Publication year - 2009

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.1187

Subject(s) - chemistry , chromatography , reagent , diastereomer , detection limit , derivatization , enantiomer , repeatability , elution , high performance liquid chromatography , chiral derivatizing agent , atenolol , chiral column chromatography , organic chemistry , medicine , blood pressure , radiology

An HPLC method was established for enantioseparation of ( R , S )‐atenolol (ATE) and determination of enantiomers in rat plasma. Marfey's reagent (1‐fluoro‐2,4‐dinitrophenyl‐5‐L‐alanine amide, FDNP‐L‐Ala‐NH 2 , MR) was used as chiral derivatizing reagent with detection of diastereomers at 340 nm. It was shown that the R ‐isomer eluted before the S ‐isomer. The method was validated for linearity, repeatability, limits of detection and limit of quantification (LOQ). Recovery of ATE at LOQ was 92.8% for ( R )‐ATE and 92.6% for ( S )‐ATE. Copyright © 2009 John Wiley & Sons, Ltd.

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