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Simultaneous determination of four antiepileptic drugs in serum by high‐performance liquid chromatography
Author(s) -
Levert H.,
Odou P.,
Robert H.
Publication year - 2002
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.117
Subject(s) - carbamazepine , chemistry , chromatography , oxcarbazepine , phenytoin , oxazepam , phenobarbital , high performance liquid chromatography , protein precipitation , anticonvulsant , acetonitrile , absorbance , pharmacology , epilepsy , benzodiazepine , biochemistry , medicine , receptor , neuroscience , biology
A high‐performance liquid chromatographic method has been developed for the simultaneous determination of oxcarbazepine, 10‐hydroxycarbamazepine, epoxycarbamazepine, carbamazepine, phenobarbital and phenytoin. After protein precipitation by acetonitrile, the supernatant was analysed on a C 18 reversed‐phase HPLC column. Antiepileptic drugs and oxazepam (internal standard) were detected by ultraviolet absorbance at 240 nm. Linearity was established for the whole concentration range for each compound. Quantitation limits of oxcarbazepine, 10‐hydroxycarbamazepine, epoxycarbamazepine, carbamazepine, phenobarbital and phenytoin were 0.58, 3.5, 2.35, 0.66, 1.02 and 3.13 µg/mL, respectively, and mean recoveries added to serum were 105.15, 84.76, 94,45, 96.52, 98.62 and 95.08%, respectively. This method has been used for the simultaneous determination of steady‐state serum concentration of antiepileptic drugs in patients treated by one or more anticonvulsive treatment. Copyright © 2001 John Wiley & Sons, Ltd.