Premium
Stereospecific high‐performance liquid chromatography of taxifolin, applications in pharmacokinetics, and determination in tu fu ling ( Rhizoma smilacis glabrae ) and apple ( Malus × domestica )
Author(s) -
VegaVilla Karina R.,
Remsberg Connie M.,
Ohgami Yusuke,
Yáñez Jaime A.,
Takemoto Jody K.,
Andrews Preston K.,
Davies Neal M.
Publication year - 2009
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1165
Subject(s) - taxifolin , chemistry , chromatography , enantiomer , high performance liquid chromatography , stereospecificity , detection limit , pharmacokinetics , standard curve , stereochemistry , biochemistry , pharmacology , antioxidant , medicine , flavonoid , catalysis
A stereospecific method of analysis of racemic taxifolin (+/−3,5,7,3′,4′‐pentahydroxyflavanone) in biological fluids is necessary to study pharmacokinetics and disposition in fruit and herbs. A simple high‐performance liquid chromatographic method was developed for the determination of all four taxifolin enantiomers. Separation was achieved on a Chiralcel® OJ‐RH column with UV detection at 288 nm. The standard curves in serum were linear over a range of 0.5–100.0 µg/mL for each enantiomer. The mean extraction efficiency was >88.0%. Precision of the assay was <15% (CV), and was within 12% at the limit of quantitation (0.5 µg/mL). The bias of the assay was <15%, and was within 6% at the limit of quantitation. The assay was successfully applied to stereospecific disposition of taxifolin enantiomers in rats and to the quantification of taxifolin enantiomers in tu fu ling ( Rhizoma smilacis glabrae ) and apple ( Malus × domestica ). Copyright © 2009 John Wiley & Sons, Ltd.