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Chiral separation of naphthalene‐2, 3‐dialdehyde labelled peptides by cyclodextrin‐modified electrokinetic chromatography
Author(s) -
DeSilva Kapila,
Jiang Qun,
Kuwana Theodore
Publication year - 1995
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130090624
Subject(s) - chemistry , chromatography , cyclodextrin , naphthalene , electrokinetic phenomena , micellar electrokinetic chromatography , organic chemistry , electrophoresis
The chiral separation of several dipeptide enantiomers, derivatized by naphthalene‐2,3‐dialdehyde (NDA), was achieved by use of cyclodextrin‐modified micellar electrokinetic chromatography (CD‐MEKC). The dipeptides contained one or two chiral centers. In the case of several dipeptides containing two chiral centers, all four of the optical isomers could be separated and baseline resolved in less than 15 min with 20 m M γ‐CD and 50 m M sodium dodecyl sulphate (SDS) in the background electrolyte. The order of elution of these derivatized dipeptides was explained by considering important chemical equlibria in the SDS, CD and peptide system. Additionally, the influence of geometric location of the chiral center, the structure of the side‐chain and the effect of CD concentration on the resolution are discussed.