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Simultaneous determination of catecholamines, their basic metabolites and serotonin in urine by high‐performance liquid chromatography using A mixed‐mode column and an eight‐channel electrochemical detector
Author(s) -
Mashige Fumiko,
Matsushima Yoshikazu,
Miyata Chizuko,
Yamada Rika,
Kanazawa Hideko,
Sakuma Ichiro,
Takai Nobuharu,
Shinozuka Noriko,
Ohkubo Akiyuki,
Nakahara Kazuhiko
Publication year - 1995
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130090506
Subject(s) - chemistry , chromatography , normetanephrine , metanephrine , urine , tyramine , detection limit , serotonin , norepinephrine , dopamine , endocrinology , biochemistry , medicine , receptor
A high‐performance liquid chromatographic method for simultaneous determination of free catecholamines, their basic metabolites and serotonin in human urine was developed. The compounds were separated on a precolumn of cation‐exchange resin and a mixed‐mode (C 18 /cation‐exchange) column and determined by an eight‐channel electrochemical detector. By this method, free norepinephrine (NE), epinephrine (E), dopamine (DA), normetanephrine (NMN), metanephrine (MN), 3‐methoxy‐tyramine (3‐MT) and serotonin (5‐HT) were determined in 40 min with inter‐assay precision of 1.9, 3.2, 1.2, 1.6, 1.6, 5.5 and 3.2%, respectively. Detection limits were between 0.05 (E) and 0.3 (3‐MT) pmol. The mean values of analytical recovery for NE, E, DA, NMN, MN, 3‐MT, 5‐HT were 99, 104, 104, 99, 102, 109 and 99% respectively.