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TLC characterization of liposomes containing D ‐myo‐inositol derivatives
Author(s) -
Brailoiu Eugen,
Huhurez Gabi,
Slatineanu Sebastian,
Filipeanu Catalin M.,
Costuleanu Marcel,
Branisteanu Dimitrie D.
Publication year - 1995
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130090405
Subject(s) - liposome , chemistry , phosphatidylcholine , chromatography , inositol , silica gel , distilled water , inositol phosphate , biochemistry , phospholipid , receptor , membrane
The thin‐layer chromatographic (TLC) behaviour of liposomes containig inositol phosphates (IPs) was studied. The liposomes contained different concentrations of D ‐myo‐inositol 1, 4, 5‐trisphosphate (IP 3 ), D ‐myo‐inositol 1, 2, 6‐trisphosphate (α‐trinositol, PP 56, a novel Perstorp Pharma derivative), D ‐myo‐inositol 1, 3, 4, 5‐tetrakisphosphate (IP 4 ), D ‐myo‐inositol 1, 3, 4, 5, 6‐pentakisphosphate (IP 5 ) and D ‐myo‐inositol 1, 2, 3, 4, 5, 6‐hexakisphosphate (IP 6 ). Migration of all liposome batches was compared to that of control liposomes (containing only triple‐distilled water), and to that of free phosphatidylcholine (PC); the same amount of lipid was used in all situations. Thin‐layer chromatography was performed on silica gel as adsorbent. As solvent we used an n ‐buthanol: ethanol: water mixture in a 4:3:3 volume ratio. Significant differences were found between PC and all liposome batches, as well as between control liposomes and the ones containing IP 3 , α‐trinositol, IP 4 , or IP 5 , in various concentrations. Liposomes containing IP 6 migrate completely differently compared not only to phosphatidylcholine and control liposomes, but also to the ones containing other IPs (<10 −3 M ). Unlike the other IPs studied, liposome‐entrapped IP 6 elicits dose‐independent contractions of the isolated rat aorta. This suggests that liposomes loaded with IP 6 undergo, during or after their preparation, physico‐chemical alterations that eventually change their drug‐delivery capacity.