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Determination of argininosuccinate lyase in human serum by ion‐pair reversed phase liquid chromatography
Author(s) -
Canepari S.,
Carunchio V.,
Girelli A. M.,
Messina A.
Publication year - 1995
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130090404
Subject(s) - chemistry , chromatography , argininosuccinate lyase , argininosuccinate synthase , detection limit , enzyme , high performance liquid chromatography , biochemistry , arginase , arginine , amino acid
A new assay for argininosuccinate lyase based on the separation of the enzymatic reaction mixture components by an ion‐pair reversed phase mechanism is reported. The determination of enzyme activity is performed after direct injection by UV detection of the fumarate formed. The chromatographic analysis time is 8 min and a detection limit of 0.4 U/L is achieved. The HPLC method is highly accurate, sensitive and precise. The simple procedure makes this method suitable for the routine determination of ASAL activity in human serum samples.