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The development of a sensitive myo‐inositol analyser using a liquid chromatograph with a post‐label fluorescence detector
Author(s) -
Inamoto Yukiko,
Hiraga Yayoi,
Hanai Toshihiko,
Kinosita Toshio
Publication year - 1995
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130090307
Subject(s) - chemistry , inositol , chromatography , reagent , fluorescence , chronic renal failure , biochemistry , receptor , endocrinology , organic chemistry , medicine , physics , quantum mechanics
A sensitive liquid chromatographic analysis for myo‐inositol was developed using glycocyamine as the postlabelling reagent. The sensitivty was 500 pmol/injection. The system was applied to analyse myo‐inositol in sera from eight patients with chronic renal failure. The average concentration of serum myo‐inositol was 498.6 ± 257.0 μmol/L before haemodialysis, and 244.0 ± 131.1 μmol/L after haemodialysis. These results indicated that the kidney is the main site of myo‐inositol metabolism.