Premium
Simultaneous determination of nadolol enantiomers in human plasma by high‐performance liquid chromatography using fluorescence‐detection
Author(s) -
Belas Frank J.,
Phillips Mark A.,
Srinivas Nuggehally R.,
Barbhaiya Rashmi H.,
Blair Ian A.
Publication year - 1995
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130090306
Subject(s) - nadolol , chemistry , chromatography , derivatization , enantiomer , reagent , high performance liquid chromatography , detection limit , human plasma , pharmacokinetics , fluorescence , stereochemistry , propranolol , pharmacology , organic chemistry , medicine , physics , quantum mechanics
A high‐performance liquid chromatographic assay is described for the separation and quantification of nadolol isomers in human plasma. The isomers were quantified using reverse‐phase HPLC and fluorometric detection after derivatization with the chiral reagent R(‐)‐1‐(naphthyl)ethylisocyanate [ R (‐)‐NEI]. The N ‐isopropyl analogue (one isomer) of nadolol was used as the internal standard. The method was reproducible based on precision studies where the percent relative standard deviation was less than 15%. The lower limit of quantitation for each isomer was 2.5 ng/mL. This method was used to evaluate the pharmacokinetic profile of nadolol isomers in human subjects following both single and multiple oral dosing.