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Direct separation of tranylcypromine enantiomers and their profile in an atypical depressive patient
Author(s) -
AboulEnein Hassan Y.,
Serignese Vince
Publication year - 1995
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130090209
Subject(s) - chemistry , enantiomer , chromatography , resolution (logic) , tranylcypromine , perchloric acid , methanol , capacity factor , phase (matter) , diastereomer , high performance liquid chromatography , organic chemistry , monoamine oxidase , artificial intelligence , computer science , enzyme
Abstract An isocratic and simple high‐performance liquid chromatographic method is developed for the diirect resolution of the tranylcypromine (TCP) enatiomers. The method involves the use of an S‐18‐crown‐6‐ether chiral stationary phase known as the Crownpak CR (+) column. The stereochemical separation factor (α) obtained was 1.30 and the stereochemical resolution factor ( R s ) was 0.69 when using a mobile phase consisting of 0.1 N perchloric acid containing 12% methanol at 23°C. The method has been used to monitor and identify, qualitatively, the profile of enantiomers of TCP in urine of an atypical depressive patient. It was found that the (−)‐ S ‐TCP concentration is significantly higher than the (+)‐ R ‐enantiomer.