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A highly sensitive method to quantify triazolam and its metabolites with liquid chromatography—mass spectrometry
Author(s) -
Senda Naoto,
Kohta Kimiyoshi,
Takahashi Toshiaki,
Shizukuishi Kenichi,
Mimura Tadao,
Fujita Tomoyuki,
Nakayama Mitsuru
Publication year - 1995
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130090110
Subject(s) - chemistry , chromatography , triazolam , mass spectrometry , ammonium acetate , methanol , extraction (chemistry) , chemical ionization , solvent , urine , solid phase extraction , sample preparation , high performance liquid chromatography , ionization , ion , benzodiazepine , biochemistry , receptor , organic chemistry
We established a highly sensitive method to determine triazolam and its major metabolites, α‐hydroxytriazolam and 4‐hydroxytriazolam, in human plasma and urine with a liquid chromatography‐mass spectrometry system which incorporates an atmospheric chemical ionization interface. A plasma sample and a urine sample after solvent extraction were injected into an ODS column of reversed phase with a mobile phase in a linear solvent gradient of initially 50 m M ammonium acetate (pH 4.0) 50%: methanol 50% and 15 min later methanol 100%; quantification limits of as low as 20 pg/mL at an SNR of 3 were obtained for each compound. With diazepam as an internal standard, recovery yields of 84, 81, and 77% were obtained for triazolam, α‐hydroxytriazolam and 4‐hydroxytriazolam, respectively.