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Solid phase extraction and high performance liquid chromatographic determination of dobutamine in plasma of dialysed patients
Author(s) -
Leflour C.,
Dine T.,
Luyckx M.,
Brunet C.,
Gressier B.,
Cazin M.,
Robert H.,
Durocher A.,
Cazin J. C.
Publication year - 1994
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130080613
Subject(s) - chromatography , chemistry , dobutamine , detection limit , extraction (chemistry) , cartridge , reproducibility , solid phase extraction , high performance liquid chromatography , acetonitrile , medicine , materials science , hemodynamics , metallurgy
An isocratic reversed‐phase high performance liquid chromatographic method has been developed for the determination of dobutamine in the plasma of dialysed patients. A solid phase extraction method with a Sep‐Pak C 18 cartridge was used to isolate the drug and isoxsuprine (internal standard) from plasma. The separation was carried out on an ODS‐Hypersil column with 0.1 M phosphate buffer: acetonitrile: methanol (72 : 20 : 8 v/v/v) as the mobile phase. The recovery of dobutamine added to plasma by the extraction procedure was 87 ± 2.3% (mean ± SD). The accuracy and reproducibility of the method were within acceptable limits over the concentration range 0–1000 ng/ mL. Quantification was by fluorescence detection at 275 nm excitation and 310 nm emission wavelengths with a detection limit of 5 ng/ mL for dobutamine. This procedure was applied to ascertain the pharamacokinetics of dobutamine infusion in nine patients with cardiogenic shock and end‐stage renal disease undergoing haemodialysis.