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High‐resolution separation of PCR product and gene diagnosis by capillary gel electrophoresis
Author(s) -
Baba Yoshinobu,
Tomisaki Riyo,
Sumita Chinuyo,
Tsuhako Mitsutomo,
Miki Tetsuro,
Ogihara Toshio
Publication year - 1994
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130080608
Subject(s) - capillary electrophoresis , chromatography , chemistry , genotyping , gel electrophoresis , gel electrophoresis of nucleic acids , electrophoresis , polymerase chain reaction , dna , capillary action , restriction fragment , resolution (logic) , free flow electrophoresis , microbiology and biotechnology , polyacrylamide gel electrophoresis , gene , gel electrophoresis of proteins , biology , biochemistry , genotype , materials science , artificial intelligence , computer science , composite material , enzyme
High‐resolution separation of a PCR product from a mixture of DNA restriction fragments was achieved using capillary gel electrophoresis. The capillary gel electrophoretic separation gives an excellent resolution of two fragments of the 500‐bp PCR product and the 506‐bp DNA fragment, which differs by only 6 bp, and the complete separation of a broader chain lenght range of DNA fragments up to 12kbp within 20min. The plate number of gel‐filled capillary was achieved to be 2 million plates per meter. Capillary gel electrophoresis is applied to the gene diagnosis for heart disease through apolipoprotein E genotyping. The advantages and limitations of capillary gel electrophoresis in the application to PCR analysis and DNA diagnosis are discussed.