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Determination of fluvoxamine concentration in plasma by reversed‐phase liquid chromatography
Author(s) -
Wong Steven H. Y.,
Kranzler Henry R.,
Dellafera Sandra,
Fernandes Rosinda
Publication year - 1994
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130080605
Subject(s) - chemistry , fluvoxamine , chromatography , pharmacokinetics , desipramine , phosphoric acid , isoamyl alcohol , high performance liquid chromatography , extraction (chemistry) , pharmacology , alcohol , serotonin , medicine , biochemistry , fluoxetine , receptor , organic chemistry , hippocampus , antidepressant
Fluvoxamine, a serotonin re‐uptake inhibitor, was quantified in plasma by modifying a previously published procedure for monitoring plasma concentrations of tricyclic antidepressants. Alkalinized plasma samples were extracted with n ‐hexane/isoamyl alcohol, followed by back‐extraction with diluted phosphoric acid, The extracts were analysed by reversed‐phase liquid chromatography using a C‐18 column, with phosphate/acetonitrile as the mobile phase. The assay was linear from 10 to 800 μg/L. Precision studies showed within‐run and day‐to‐day coefficients of variation to be 4.5 and 6.8%, respectively. Desipramine interfered with the detection of fluvoxamine. The assay was used to measure a total of 8 plasma samples from 4 alcohol‐dependent patients medicated with fluvoxamine as an adjunct to relapse prevention psychotherapy. In these patients, the plasma concentrations ranged from 54 to 241 μg/L. Dosage of fluvoxamine, duration of treatment, interval between last dosage and blood collection were associated with effects on plasma concentrations that were consistent with the pharmacokinetic profile of fluvoxamine.