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Measurement of amiloride in airway surface liquid utilizing HPLC and fluorescence detection
Author(s) -
Selinger Krzysztof
Publication year - 1994
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130080505
Subject(s) - chemistry , amiloride , chromatography , triamterene , high performance liquid chromatography , methanol , fluorescence , extraction (chemistry) , acetonitrile , phosphate buffered saline , analytical chemistry (journal) , sodium , medicine , physics , organic chemistry , quantum mechanics , blood pressure , hydrochlorothiazide , radiology
A high‐performance liquid chromatographic method for the determination of amiloride in airway surface liquid is described. It involves extraction of the drug with methanol from filter paper on which the sample is absorbed and chromatography on a Zorbax Rx column; the mobile phase is 25% acetonitrile in 0.05 M phosphate buffer; detection by fluorescence at 360/420 nm. Triamterene is used as an internal standard. The range of the assay is 2.0–2033.4 ng/sample, with adequate precision and accuracy. A power curve y = ax b best describes the relationship between the peak‐height ratio and concentration. Recovery of amiloride is non‐linear, probably due to an adsorption process. Accuracy of the assay at lower amounts may be affected by the choice of filter paper a well as by presence of endogenous plasma components. The assay was used to measure amiloride amount in airway surface liquid after administration of 2.5 and 4.5 mg of nebulized amiloride.