Premium
Separation of vitamin B complex and folic acid using TLC plates impregnated with some transition metal ions
Author(s) -
Bhushan Ravi,
Parshad Vineeta
Publication year - 1994
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130080411
Subject(s) - chemistry , vapours , ammonia , butanol , chromatography , folic acid , silica gel , metal ions in aqueous solution , propanol , solvent , inorganic chemistry , nuclear chemistry , ion , ethanol , organic chemistry , medicine , neuroscience , biology
The separation of Vitamin B complex and folic acid was achieved on silica gel layers impregnated with Mn ++ , Fe ++ , Co ++ , Ni ++ , Cu ++ , Cd ++ , Zn ++ or Mg ++ (0.1%, 0.2%, 0.3%, 0.4% of each ion). Three new solvent systems n ‐propanol: n ‐butanol: water: ammonia (7:5:1:2, by vol), n ‐propanol: n ‐butanol: water: ammonia (7:5:1:1.5, by vol), n ‐propanol: n ‐butanol: water: ammonia (7:5:0.75:2, by vol) were developed. The spots of vitamins were located by exposing the chromatoplate to iodine vapours.