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TLC—A rapid method for liposome characterization
Author(s) -
Brailoiu Eugen,
Saila Liliana,
Huhurez Gabi,
Costuleanu Marcel,
Filipeanu Catalin M.,
Slatineanu Sebastian,
Cotrutz Carmen,
Branisteanu Dimitrie D.
Publication year - 1994
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130080410
Subject(s) - chemistry , liposome , chromatography , phosphatidylcholine , vesicle , chloroform , alcohol , thin layer chromatography , butanol , solvent , ethanol , membrane , organic chemistry , biochemistry , phospholipid
One of the most important problems for the use of liposomes as a drug delivery system is the modification of the vesicle induced by the liquid medium in which they are introduced (blood plasma for in vivo studies and the saline buffer solution for in vitro studies). Using thin‐layer chromatography (TLC) we compared the behaviour of phosphatidylcholine (used for liposomes preparation) to that of the following unfilled liposomes: multilamellar liposomes (MLV); small unilamellar vesicles (SUV); and reverse phase evaporation vesicles (REV), before and after storage for 15 min in Krebs‐Henseleit solution (37°C, pH 7.4, aereated continuously with 95% O 2 + 5% CO 2 ). All variants contained the same amount of phosphatidylcholine. Thin‐layer chromatography was performed on silica gel 60 as adsorbant. Two types of solvents were tested: one based on chloroform/alcohol ( n ‐butanol or n ‐propanol or methanol)/water mixture (in different ratios) and another based on alcohol/alcohol/water mixture ( n ‐butanol/ n ‐propanol/water in 4/3/3 volume ratio). In all variants of chloroform containing solvents no differences were found between phosphatidylcholine and all types of liposomes. When using as solvent n ‐butanol/ n ‐propanol/water significant differences were found between all types of liposomes before and after storage in Krebs‐Henseleit solution. Their presence, after TLC treatment, was shown in electron microscopy studies.