A new matrix for affinity chromatography and its application in the separation of a human monoclonal antibody | Zendy

Guo Wei | Zendy; Shang Zhenhua | Zendy; Yu Yinian | Zendy; Zhou Liangmo | Zendy

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A new matrix for affinity chromatography and its application in the separation of a human monoclonal antibody

Author(s) -

Guo Wei,

Shang Zhenhua,

Yu Yinian,

Zhou Liangmo

Publication year - 1994

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.1130080310

Subject(s) - chemistry , chromatography , sepharose , monoclonal antibody , concanavalin a , affinity chromatography , bovine serum albumin , trypsin , matrix (chemical analysis) , enzyme , antibody , biochemistry , biology , immunology , in vitro

A method for the preparation of a p ‐aminobenzene sulphonyl ethyl containing crosslinked Sepharose 4B (ABSE‐Sepharose 4B‐CL) is described. trypsin, bovine serum albumin (BSA) and concanavalin A (Con A) were immobilized onto this matrix by diazotization. Conditions for the coupling reaction were investigated. The activity of immobilized trypsin reached 1.4 × 10 4 U/g, and 25 mg BSA can be coupled onto 1 g ABSE‐Sepharose 4B‐CL under the optimal conditions. An affinity medium with immobilized Con A as ligand was prepared by this method, and was used in the separation of a human monoclonal antibody.

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