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A new matrix for affinity chromatography and its application in the separation of a human monoclonal antibody
Author(s) -
Guo Wei,
Shang Zhenhua,
Yu Yinian,
Zhou Liangmo
Publication year - 1994
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130080310
Subject(s) - chemistry , chromatography , sepharose , monoclonal antibody , concanavalin a , affinity chromatography , bovine serum albumin , trypsin , matrix (chemical analysis) , enzyme , antibody , biochemistry , biology , immunology , in vitro
A method for the preparation of a p ‐aminobenzene sulphonyl ethyl containing crosslinked Sepharose 4B (ABSE‐Sepharose 4B‐CL) is described. trypsin, bovine serum albumin (BSA) and concanavalin A (Con A) were immobilized onto this matrix by diazotization. Conditions for the coupling reaction were investigated. The activity of immobilized trypsin reached 1.4 × 10 4 U/g, and 25 mg BSA can be coupled onto 1 g ABSE‐Sepharose 4B‐CL under the optimal conditions. An affinity medium with immobilized Con A as ligand was prepared by this method, and was used in the separation of a human monoclonal antibody.