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Glutathione and cysteine measurement in biological samples by HPLC with a glassy carbon working detector
Author(s) -
Carvalho F. D.,
Remião F.,
Valet P.,
Timbrell J. A.,
Bastos M. L.,
Ferreira M. A.
Publication year - 1994
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130080308
Subject(s) - chemistry , chromatography , cysteine , glutathione , high performance liquid chromatography , detector , glassy carbon , carbon fibers , biochemistry , optics , enzyme , cyclic voltammetry , physics , materials science , electrode , composite material , composite number , electrochemistry
An HPLC method using a glassy carbon working detector for measuring cysteine and reduced glutathione (GSH) in biological samples was developed. Oxidized glutathione (GSSG) was also measured after reduction with glutathione reductase. This study was conducted in human plasma. Cysteine and GSH standard curves were linear in the physiological range, presenting detection limits of 22.0 pmol and 6.0 pmol respectively. Plasmatic results found were 43.92 ± 4.15, 4.50 ± 0.65, and 0.19 ± 0.12 m M (means ± SE), for cysteine, GSH and GSSG, respectively. Peak specificity for cysteine and reduced glutathione was certified by their disappearance after treatment with N ‐ethylmaleimide.