Premium
Determination of sulphur amino acids by gas chromatography with flame photometric detection
Author(s) -
Kataoka Hiroyuki,
Tanaka Hirofumi,
Fujimoto Ayumi,
Noguchi Ikuko,
Makita Masami
Publication year - 1994
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130080305
Subject(s) - chemistry , chromatography , amino acid , sulfur , gas chromatography , calibration curve , urine , detection limit , organic chemistry , biochemistry
A selective and sensitive method has been developed for the determination of sulphur amino acids by gas chromatography (GC). Sulphur amino acids were converted into their N ( S )‐isopropoxycarbonyl methyl ester derivatives and measured by GC with flame photometric detection using a DB‐17 capillary column. The derivatives were sufficiently volatile and stable to give single symmetrical peaks. The detection limits of sulphur amino acids were ca. 0.5–1 pmol per injection, and the calibration curves were linear in the range 0.5–1 nmol for each sulphur amino acid. This method was successfully applied to small urine samples without prior cleanup, and sulphur amino acids in these samples could be analysed without any influence from coexisting substances. Overall recoveries of sulphur amino acids added to urine samples were 85–113%. The analytical results of free sulphur amino acid contents in urine samples of normal subjects are presented.