Premium
UV‐transparent, replaceable agarose gels for molecular‐sieve (capillary) electrophoresis of proteins and nucleic acids
Author(s) -
Hjertén Stellan,
Srichaiyo Tasanee,
Palm Anders
Publication year - 1994
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130080206
Subject(s) - agarose , chemistry , capillary electrophoresis , chromatography , nucleic acid , capillary action , electrophoresis , gel electrophoresis of nucleic acids , polyacrylamide , agarose gel electrophoresis , dna , polymer chemistry , biochemistry , materials science , composite material
Gels of methoxylated agarose (gelling point 25.6 °C) and other low‐melting agarose derivatives compare favorably with cross‐linked polyacrylamide gels for capillary and slab molecular‐sieve electrophoresis of proteins and DNA. These agarose gels can be pressed out of the capillary following a run and replaced by an agarose solution with a temperature of 35–40 °C. Gelation occurs upon lowering the temperature and the same capillary can thus be reused for another analysis with a fresh gel. The methoxylated, non UV‐absorbing agarose gels are, accordingly, replaceable, which makes them very attractive for series analyses with modern, automated capillary electrophoresis apparatus. The high resolution of these agarose gels is demonstrated with a separation of an albumin sample into monomers, dimers, trimers, tetramers, pentamers, hexamers, heptamers, and of DNA fragments.