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Experimental models for optimization of insulin separation on reversed phase columns
Author(s) -
Dimov N.,
Simeonov S.
Publication year - 1994
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130080109
Subject(s) - chemistry , chromatography , separation (statistics) , phase (matter) , insulin , chromatographic separation , high performance liquid chromatography , organic chemistry , computer science , medicine , machine learning
Experimentally defined relationships have been found to describe adequately the retention and separation of bovine and porcine insulins as well as their desamido products on reversed phases C 4 30 nm and C 18 10 nm pore diameter. The equations are valid for a region of initial acetonitrile concentrations from 16 to 31% and gradient rates from 0.04 to 0.60%/min. The peak heights showed an exact non‐linear relationship with the time interval between the first and last peak of interest, independent of their retention time. The number of experiments required for obtaining the parametric estimates of the models depends on the particular task, but in all cases is less than eight. The relationships found permit the correct choice to be made in advance both for analysis conditions for a particular column and for different requirements of insulin analysis.